New Paper Published “On-chip analysis of glycolysis and mitochondrial respiration in human induced pluripotent stem cells”

Our ESR Stefanie Fuchs (ESR14) together with her supervisor Torsten Mayr (Graz University of Technology) as well as our project partners Christine Mummery and Valeria Orlova (Leiden University Medical Center) and others have published a paper on “On-chip analysis of glycolysis and mitochondrial respiration in human induced pluripotent stem cells”.

The paper deals with in-situ metabolic analysis of dynamic human induced pluripotent stem cell cultures with integrated oxygen and pH sensors.

Read more here.

New Paper Published “A microfluidic chip carrier including temperature control and perfusion system for long-term cell imaging”

Our ESR Gabriel Werr (ESR8) together with his supervisor Maria Tenje and others have published a paper presenting an integrated system that enables repeated transfers of microfluidic chips used for cell culture between an incubator and a microscope whilst maintaining a physiological temperature and periodic cell media turnover.

Read more about this versatile tool for microfluidic users in need of a cost-effective platform here.

Annual Conference of the European Organ-on-Chip Society (EUROoCS) 2022

With a participant count of nearly 500 people, EUROoCS 2022 was a great success. The event spanned over two days of intense discussions, four parallel sessions with topics covering (multi)-organ and disease models, organoids-on-chip, read-out assays, standardization and qualification, among many others. A key takeaway of the conference was the recognition and discussion around standardization and validation of OoCs with the intention of the principles of 3Rs (reduction, replacement, and refinement of animal testing). We are especially proud of our ESRs Alessia (oral), Tarek (oral), Stefanie (oral), Lisette (oral), Kenny (poster), Merve (poster), Mara (poster), Joanna (poster), Tanvi (poster), Anders (poster) and Gabriel (poster) who presented their great achievements in the course of the conference.

EUROoC M44 Meeting

Already one day prior to the start of the EUROoCS Conference 2022, the EUROoC ITN consortium met on 3 July 2022 in Grenoble, France for their bi-annual consortium meeting. The day was kicked off by a meeting of the EUROoC PIs who recapitulated the project, its challenges but also its great successes. Especially considering the massive restrictions caused by the Covid-19 pandemic, all beneficiaries found it particularly impressive how well the ESRs nevertheless worked together, maintained the exchange and built up their own professional network, from which they will undoubtedly benefit massively in their further professional careers. After this discussion forum, the ESRs joint the meeting and presented the progress they made during the last three years on their individual projects and explained how collaborations with each other enabled better development of their systems.

Finally, after a long day of great Organ-on-Chip science, the EUROoC consortium got to enjoy some delicious French cuisine during the conference dinner. It has been great to finally be able to meet everyone in person and to conclude the meeting in good company.

New Paper Published on “Fusing spheroids to aligned μ-tissues in a heart-on-chip featuring oxygen sensing and electrical pacing capabilities”

Our ESR Alessia Moruzzi (ESR1) and Stephanie Fuchs (ESR14) together with ther supervisors and others have published a paper presenting a novel cell injection and tissue generation concept relying on spheroids, which can be produced in large quantities and uniform size from induced pluripotent stem cell-derived human cardiomyocytes. 

Read more about their interesting work and results here.

New Paper Published on “Microphysiological stem cell models of the human heart”

Our project partners from the Natural and Medical Sciences Institute at the University of Tübingen (NMI), the Leiden University Medical Center (LUMC) and Miltenyi Biotech (MTY) have published a paper on the “Microphysiological Stem Cell Models of the Human Heart”.Models of heart disease and drug responses are increasingly based on human pluripotent stem cells (hPSCs) since their ability to capture human heart (dys-)function is often better than animal models. Simple monolayer cultures of hPSC-derived cardiomyocytes, however, have shortcomings. Some of these can be overcome using more complex, multi cell-type models in 3D. Here we review modalities that address this, describe efforts to tailor
readouts and sensors for monitoring tissue- and cell physiology (exogenously and in situ) and discuss perspectives for implementation in industry and academia. Read more here.

ESR 8 – Secondment in Luxembourg

Hi, my name is Gabriel Werr (ESR 8) and I am researching “Microfluidic methods for bead-based assays on-chip” as well as on-chip sensing methods at Uppsala University.

My colleague Mara Lucchetti (ESR 12) is researching the integration of iPSC-derived epithelial cells and on-chip TEER (transepithelial electrical resistance) measurements in the HuMiX gut model. Together we have been developing Platinum thin-film electrodes that can be integrated into her existing HuMiX model without compromising the seal of her chip.

The goal of my secondment at University of Luxembourg was to set up the new TEER electrodes for continuous readout of up to eight sensors. This included choosing a suitable electrical connection method that can be sterilised while not being too time consuming during assembly, and in order to read out the eight sensors continuously a multiplexer was set up together with an automation script that switches and logs all the sensors.
Due to COVID-19 restrictions we had to resort to video conferences and exchanging samples and prototypes by mail.

With my engineering background it was very useful to learn about all the small hurdles that need to be considered when implementing new equipment in a cell experiment to make it actually usable and convenient. Mara has done a great job providing me that insight even with the added difficulty of the entire secondment being held online.

ESR Workshop CV Writing & Interviewing Techniques

On 4 April the ESRs attended the virtual workshop about “CV Writing & Interviewing Techniques” by Matthias Nagel. They learned how to make their CV stand out by giving a personal touch to the layout and how to use professional online profiles to support the CV. Additionally, they were introduced to several websites that could help them understand the company’s reputation, and the candidate’s market value (i.e. Glassdoor, Stepstone etc.). In the second half of the workshop, experienced principal investigators and recruiters from the ITN network conducted a panel discussion to elaborate on what they looked for during interviews and in candidates’ resumés. The ESRs found it particularly useful, as an exercise, to review each other CVs in order to gain a new perspective and to improve their CV format. As the last training workshop of the ITN, we hope that the ESRs can confidently progress towards their next steps in academia or industry.

New Paper Published on the study of metabolism using multi-organ chips

Our project partners from the Natural and Medical Sciences Institute at the University of Tübingen (NMI) and Jena University Hospital (JUH) have published a paper on the “Studying metabolism with multi-organ chips: new tools for disease modelling, pharmacokinetics and pharmacodynamics”.

Non-clinical models to study metabolism including animal models and cell assays are often limited in terms of species translatability and predictability of human biology. This field urgently requires a push towards more physiologically accurate recapitulations of drug interactions and disease progression in the body. Multi-organ chips (MOCs) are an emerging technology that is well suited to providing a species-specific platform to study the various types of metabolism by recreating organ-level function. This review provides a resource for scientists aiming to study human metabolism by providing an overview of MOCs recapitulating aspects of metabolism, by addressing the technical aspects of MOC development and by providing guidelines for correlation with in silico models. Read more here.